TetX antibody

Catalog No. ABIN7232855

The Mouse Monoclonal anti- antibody has been validated for ELISA, WB, InhA and Neut. It is suitable to detect in samples from Clostridium tetani.

Quick Overview for TetX antibody (ABIN7232855)

Target: TetX

Reactivity: Clostridium tetani

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB), Inhibition Assay (InhA), Neutralization (Neut)

Clone: B416M

Product Details

Purpose: Anti-Clostridium tetani Toxin Mouse Monoclonal Antibody

Specificity: This antibody recognizes C. tetani toxin.

Cross-Reactivity: Clostridium tetani

Immunogen: Toxin purified from culture filtrate of C. tetani.

Isotype: IgG1

Application Details

Application Notes: ELISAImmunoblotting A band of ~150,000 is detected.
End users should determine optimal dilutions for their applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Concentration: Lot specific

Buffer: PBS, pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: This antibody is stable at 4°C for short-term storage and for at least one (1) year at -20° to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

Target Details

Target: TetX

Background: Tetanus toxin,Tetanus toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the '76-Gln-|-Phe-77' bond of synaptobrevin-2.,Clostridium tetani is an anaerobic, gram-positive, spore-forming rod found in soil and in the intestinal tracts and feces of animals. Most cases of tetanus result C. tetani spores entering small puncture wound. Infections remain localized, but systemic damage results from the production of exotoxins that are released when the spores germinate and grow. Tetanus toxin â€Tetanospasmin†is one of the most potent toxins known, third only to botulinum toxin and diphtheria toxin. This potent neurotoxin enters peripheral nerves and travels to the central nervous system where it interferes with the release of neurotransmitters, causing muscle contraction and spasm.

UniProt: P04958